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Strengthening awareness and education to address food safety problem is of importance. The purpose of this study is to investigate food safety knowledge and opinion among Serbian students and the efficiency of education on their self-reported behaviour as consumers.

A questionnaire on the attitudes and self-reported behaviour of students was completed by 414 students at the Faculty of Agriculture, University of Belgrade, Serbia. The questions covered hand hygiene; cross-contamination; conditions of food cooling; and knowledge of risky food. Students were divided into two groups: students in their first and second year of study (1–2 YoS) who had not attended any subjects related to food safety during their education; students in the third and fourth years of the Food Technology Program (3–4 YoS) who had completed one or more courses concerning food safety during their education.

Overall, there were significant differences in the opinions of 1–2 YoS students and 3–4 YoS students on most issues. It was noted that male students are at higher risk when it comes to food handling. Considering the significant impact of education as evidenced by the opinions of 3–4 YoS students, it can be concluded that education is effective in raising awareness and changing behaviour among young people.

The study identified how education affects students' opinion and food handling. Moreover, it highlighted the areas of deficiency in students' food safety behaviour, knowledge and attitudes. Limited research has been conducted on food safety knowledge among students.